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KMID : 0380020060210060493
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 6 p.493 ~ p.497
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Two Stage Fermentation of Xylose/Glucose Mixture for Xylitol Production by Candida mogii
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Baek Seong-Chul
Kwon Yun-Joong
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Abstract
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Two stage fermentations of glucose/xylose mixture which is similar composition with rice straw hemicellulose hydrolysate were performed by Candida mogii ATCC 18364. In first stage, glucose was consumed rapidly for cell growth in aerobic condition (2 vvm, 300 rpm), then D-xylose was used for xylitol production in semi-aerobic condition (1 vvm, 300 rpm). After 4 days of fermentation, about xylitol was produced with a yield of 0.58 g/g and volumetric productivity of . To improve the xylitol yield by reduction of xylose consumption for cell growth and maintenance, D-glucose was continuously supplemented during the second stage of fermentation. By D-glucose feeding of day, xylitol was produced up to with a yield of 0.8 g/g and volumetric productivity which are 1.2-1.3 times higher than those obtained without D-glucose feeding.
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KEYWORD
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Xylitol, xylose/glucose, Candida mogii, glucose-feeding
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